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Figure 2 | Cell Communication and Signaling

Figure 2

From: Human uterine leiomyoma-derived fibroblasts stimulate uterine leiomyoma cell proliferation and collagen type I production, and activate RTKs and TGF beta receptor signaling in coculture

Figure 2

Assessment of cell proliferation based on cell counts and PCNA labeling. (A) Cell proliferation. There was a significant (p = 0.004) increase in the number of UtLM cells cocultured with fibroblasts (UtLM/FB) compared to UtLM cells cultured alone. There was no significant difference observed between UtSMC alone and UtSMC cocultured with FB (B) PCNA labeling. The percent PCNA labeling was significantly (p = 0.04) higher in UtLM/FB compared to UtLM cells cultured alone; however, there was no significant difference for UtSMC at both conditions. (C) PCNA immunoexpression in UtLM cells and UtLM/FB. PCNA was expressed in both cocultured and non-cocultured UtLM cells, as well as UtSMC, as indicated by brown nuclear staining. There was a significant increase in PCNA expression in the UtLM/FB compared to UtLM cells cultured alone, but not for UtSMC under similar conditions. All error bars represent SEM.

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Cell Communication and Signaling

ISSN: 1478-811X