Fig. 5

Effect of FGFR3 knockdown on downstream signaling in SUM185PE cells. a, SUM185PE cells were reverse transfected with 20 nM of individual siRNAs targeting FGFR3-TACC3 fusion and wildtype FGFR3 (FW FGFR3 1–4), or wildtype FGFR3 only (W1–3), and the indicated downstream signaling proteins analysed by Western blot. b, Quantification by densitometry of (A). Data were first normalized relative to the β-actin loading control, then phosphorylated proteins were normalized relative to total protein, then data were expressed relative to the siOTP control which was arbitrarily set at 1.0. Error bars: mean ± standard error, of three biological replicates. * indicates p-value of < 0.05, ** < 0.01, *** < 0.001